Identification of a Sperm Nuclear Annulus: A Sperm DNA Anchor1

Identification of a Sperm Nuclear Annulus: A Sperm DNA Anchorl

We report the isolation and characterization of a new nuclear structure from spermatozoa of the golden Syrian hamster which we term the nuclear annulus. The nuclear annul us was located at the implantation fossa, the point at which the tail is joined to the sperm head, inside the nucleus adjacent to the inner nuclear envelope. Extraction of sperm nuclei with 2 M NaC1 and 10 mM dithiothreitol ca...

Raman Analysis of Sperm Nuclear DNA Integrity

Raman Spectroscopy was evaluated as a non-invasive method of analysis of sperm DNA and the influence of UV irradiation on the sperm. The results show that Raman Spectroscopy, combined with multivariate analysis provide the reproducible and accurate information on DNA of sperm and the effect and location of damage.

P-37: Effect of Gibberellic Acid on Sperm DNA Double Strand Integrity, Nuclear Maturity and Motility

Background: Plant growth regulators gave entry into animals and human cells through diet. Gibberellic acid (GA3) is an extensively prevalent plant growth regulator due to its use in agriculture. Therefore current study was designed to investigate the effect of GA3 compound on sperm DNA integrity, nuclear maturity and motility. Materials and Methods: To follow-up current study 12 mature male s r...

Sperm nuclear DNA fragmentation and chromatin structure in one-day-old ejaculated sperm

OBJECTIVE To evaluate sperm nuclear DNA fragmentation and chromatin structure after 18 hours'incubation at room temperature. METHODS Twenty-eight male partners who participating IVF treatment were prospectively included in this study. Ejaculated sperm count and motility were assessed. The sperm was then immediately processed by the conventional swim-up method. After utilization of some of the...

A guide to sperm DNA fragmentation testing